Gal4 driver drosophila

 · Recombinant Construct: P {mex1-GAL} A kb promoter fragment from the mex1 locus is fused upstream of sequence encoding a GAL4 driver. A kb promoter fragment from the mex1 locus is fused upstream of sequence encoding a GAL4 driver. Drives expression in the midgut. The Drosophila melanogaster Neprilysin Nepl15 is involved in lipid and. Border cell (BC) migration during Drosophila oogenesis is an excellent model for the analysis of the migratory and invasive cell behavior. Most studies on BC migration have exploited a slbo-Gal4 driver to regulate gene expression in these cells or to . The Mushroom Body of Adult Drosophila Characterized by GAL4 Drivers Yoshinori Aso,1,2 Kornelia Gru¨bel,2 Sebastian Busch,1,2 Anja B. Friedrich,1 Igor Siwanowicz,1 and Hiromu Tanimoto1,2 1Max-Planck-Institut fu¨r Neurobiologie, Martinsried, Germany 2Lehrstuhl fu¨r Genetik und Neurobiologie, Universita¨t Wu¨rzburg, Wu¨rzburg, Germany Abstract: The .

The GAL4/UAS system is commonly used in Drosophila to drive expression of a gene of interest (Brand Perrimon, ). Tissue-specific or ubiquitous enhancers drive expression of GAL4 that results in transcriptional activation from the GAL4 binding sites (UAS) to drive expression of a gene of interest, and is widely used for expression of fluorescently tagged proteins and for gene knockdown by RNAi. For study in Drosophila, the GAL4 gene is placed under the control of a native gene promoter, or driver gene, while the UAS controls expression of a target gene. GAL4 is then only expressed in cells where the driver gene is usually active. Rubin and colleagues have established a collection of 7, GAL4 driver lines in Drosophila. A different, defined fragment of genomic DNA serves as a transcriptional enhancer to control GAL4 expression in each line.

The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each. There have been many GAL4 driver lines identified that prominently label the mushroom body intrinsic neurons, which are known as “Kenyon cells. The GAL4 component is a protein that switches on genes. GAL4 alone does very little in Drosophila cells because it only recognizes a DNA.